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| 别名: | Topoisomerase II alpha | ||
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| 适用物种: | Human,Mouse,Rat | ||
| 验证应用: | WB,ICC,IHC-P,FC | ||
| 种属: | 兔多抗 | ||
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Anti-Topoisomerase II alpha antibody
产品描述This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.
产品名称Anti-Topoisomerase II alpha antibody
分子量174 kDa
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm, nucleus.
其它名称
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:100-1:200
Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC
Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
