Anti-TRAP80 antibody
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概述
- 产品描述Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
- 产品名称Anti-TRAP80 antibody
- 分子量73 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human TRAP80 181-280/651
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称MED17
CRSP77
Activator recruited cofactor 77 kDa component
Activator-recruited cofactor 77 kDa component
ARC77
Cofactor required for Sp1 transcriptional activation subunit 6
CRSP complex subunit 6
CRSP6
CRSP77
DRIP80
med17
MED17_HUMAN
Mediator complex subunit 17
Mediator of RNA polymerase II transcription subunit 17
Thyroid hormone receptor-associated protein complex 80 kDa component
Transcriptional coactivator CRSP77
Trap80
Vitamin D3 receptor-interacting protein complex 80 kDa component.
more
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应用
WB:1:500-2000
FC:1μg/Test
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Fig1: Blank control (blue line): Hela(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-TRAP80 antibody ,ilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Fig2: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
Primary: Anti-TRAP80 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
Fig3: Sample:
spleen(mouse) Lysate at 40 ug
Primary: Anti-TRAP80 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73kD
Observed band size: 75 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
