Anti-RIP2 antibody

Anti-RIP2 antibody

• 概述

• 产品描述Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

• 产品名称Anti-RIP2 antibody

• 分子量60 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Guinea Pig

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human RIP2 401-540/540

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm.

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• ​其它名称Receptor interacting protein 2
  Receptor interacting serine threonine kinase 2
  Receptor interacting serine/threonine protein kinase 2
  RICK
  RIP 2
  RIP LIKE INTERACTING CLARP KINASE
  RIPK 2
  RIPK2
  TNFRSF
  CARD 3
  CARD carrying kinase
  CARD CONTAINING ICE ASSOCIATED KINASE
  CARD containing IL 1 beta ICE kinase
  CARD containing IL1 beta ICE kinase
  CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase
  CARD containing interleukin 1 beta converting enzyme associated kinase
  CARD3
  CARDIAK
  CCK
  CLARP kinase
  GIG 30
  GIG30
  Growth inhibiting gene 30
  Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

   

  

  Fig2: Sample:
  Hela(Human) Cell Lysate at 30 ug
  Primary: Anti-RIP2 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 60 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！