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Anti-ILVBL antibody

Anti-ILVBL antibody
别名: ILVBL
适用物种: Human,Mouse,Rat,Dog,Pig,Horse  
验证应用: WB,FC  
种属: 兔多抗  
储存条件: -20℃ 
Anti-ILVBL antibody
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY6911-43R-50 μl 兔多抗 现货 1500.00 1500
ZY6911-43R-100 μl 兔多抗 现货 2500.00 2500
熔点:
密度:
储存条件: -20℃

 

Anti-ILVBL antibody

 

  • 概述

  • 产品名称Anti-ILVBL antibody

  • 分子量68 KDa

  • 种属反应性Human,Mouse,Rat,Dog,Pig,Horse

  • 验证应用WB,FC

  • 抗体类型兔多抗

  • 免疫原KLH conjugated synthetic peptide derived from human ILVBL 301-400/632

  • 偶联Non-conjugated

  • 性能

  • 形态Liquid

  • 浓度1 mg/mL.

  • 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

  • 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • 亚型IgG

  • 纯化方式affinity purified by Protein A

  • 亚细胞定位Membrane.

  •  

  • ​其它名称Acetolactate synthase homolog
    Acetolactate synthase like protein
    Acetolactate synthase-like protein
    AHAS
    ILV2H
    209L8
    IlvB (bacterial acetolactate synthase) like
    IlvB like protein
    IlvB-like protein
    Ilvbl
    ILVBL_HUMAN.

    more
  • 应用

    WB:1:500-2000
    FC:3ug/Test

  •  

    Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug
    Primary: Anti-ILVBL  at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 68kD
    Observed band size: 68kD

     

    Fig2: Blank control: HepG2.
    Primary Antibody (green line): Rabbit Anti-ILVBL antibody
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1μg /test.
    Protocol
    The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

     

    Fig3: Sample:
    Hcclm3(Human) Cell Lysate at 30 ug
    MCF-7(Human) Cell Lysate at 30 ug
    A431(Human) Cell Lysate at 30 ug
    Primary: Anti- ILVBL  at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 68 kD
    Observed band size: 68 kD

  •  

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