Anti-Prostaglandin dehydrogenase 1 antibody

Anti-Prostaglandin dehydrogenase 1 antibody

• 概述

• 产品描述Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.

• 产品名称Anti-Prostaglandin dehydrogenase 1 antibody

• 分子量29 KDa

• 种属反应性Mouse,Rat,Pig,Cow,Horse,Rabbit,Human

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1 55-160/266

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm.

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• ​其它名称HPGD
  Hydroxyprostaglandin dehydrogenase 15 (NAD)
  NAD+ dependent 15 hydroxyprostaglandin dehydrogenase
  PGDH
  PGDH_HUMAN
  PGDH1
  Prostaglandin dehydrogenase 1
  SDR36C1
  Short chain dehydrogenase/reductase family 36C,member 1
  15 hydroxyprostaglandin dehydrogenase [NAD+]
  15 PGDH
  15-hydroxyprostaglandin dehydrogenase [NAD+]
  15-PGDH
  15PGDH.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PGDH) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  LOVO(Human) Cell Lysate at 30 ug
  Primary: Anti-Prostaglandin dehydrogenase 1  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 29 kD
  Observed band size: 29 kD

   

  

  Fig3: Sample:
  SW480(Human) Cell Lysate at 30 ug
  Primary: Anti-Prostaglandin dehydrogenase 1 ( at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 29 kD
  Observed band size: 29 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！