Anti-PPP3CC antibody

Anti-PPP3CC antibody

• 概述

• 产品描述Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

• 产品名称Anti-PPP3CC antibody

• 分子量58 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PPP3CC 51-150/512

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

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• 其它名称calcineurin A gamma
  CALCINEURIN A3
  Calcineurin
  Calcineurin testis specific catalytic subunit
  Calmodulin dependent calcineurin A subunit gamma isoform
  Calmodulin-dependent calcineurin A subunit gamma isoform
  CALNA3
  CAM PRP catalytic subunit
  CAM-PRP catalytic subunit
  CNA3
  pp2b gamma
  
  PP2BC_HUMAN
  PP2Bgamma
  
  PPP3CC
  protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma)
  protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform
  Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
  testis-specific catalytic subunit.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample: A549 (Human)Cell Lysate at 40 ug
  Primary: Anti-PPP3CCat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
  Predicted band size: 58kD
  Observed band size: 58kD

   

  

  Fig2: Sample: Testis(Mouse)Lysate at 40 ug
  Primary: Anti-PPP3CCat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
  Predicted band size: 58kD
  Observed band size: 58kD

   

  

  Fig3: Sample:
  DU145(Human) Cell Lysate at 40 ug
  Primary: Anti-PPP3CC ( at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 58 kD
  Observed band size: 58 kD

   

  

  Fig4: Sample:
  Hela(Human) Cell Lysate at 40 ug
  Primary: Anti-PPP3CC  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 58 kD
  Observed band size: 58 kD

   

  

  Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！