Anti-Keratan Sulfate antibody
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概述
- 产品描述Catalyzes the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
- 产品名称Anti-Keratan Sulfate antibody
- 分子量45 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Keratan Sulfate 251-350/411
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Golgi apparatus membrane.
- 其它名称Carbohydrate chondroitin 6 keratan sulfotransferase 1
Carbohydrate chondroitin 6 keratan sulfotransferase
Carbohydrate keratan sulfate Gal 6 sulfotransferase
Carbohydrate keratan sulfate Gal 6 sulfotransferase 1
Carbohydrate sulfotransferase 1
CHST1
EC 2.8.2.21
Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1
GST 1 antibody Keratan sulfate Gal 6 sulfotransferase
KS6ST
KSGAL6ST
KSST.
more
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应用
WB:1:500-2000
FC:1μg/Test
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Fig1: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Keratan Sulfate at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Fig2: Blank control(blue):RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Keratan Sulfate antibody(, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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