Anti-HNF1A antibody

Anti-HNF1A antibody

• 概述

• 产品描述Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

• 产品名称Anti-HNF1A antibody

• 分子量67 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Zebrafish,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human HNF1 201-350/628

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=P20823-1; Sequence=Displayed; Name=B; IsoId=P20823-2; Sequence=VSP_002250, VSP_002251; Name=C; IsoId=P20823-3; Sequence=VSP_002252, VSP_002253.

   

• 其它名称HNF1A
  TCF1
  HNF1 alpha
  HNF-1 Alpha
  Hepatocyte nuclear factor 1 Alpha
  Albumin proximal factor
  Hepatic nuclear factor 1 alpha
  Hepatic nuclear factor 1
  Hepatic transcription factor 1 alpha
  Hepatic transcription factor 1
  HNF 1
  HNF 1A
  HNF1A
  Interferon production regulator factor
  LF B1
  LF B1 hepatic nuclear factor
  LFB 1
  LFB1
  LFB1 hepatic nuclear factor
  Liver specific transcription factor LF B1
  Liver specific transcription factor LFB1
  Maturity onset diabetes of the young 3
  MODY 3
  MODY3
  TCF 1
  TCF1
  TCF-1
  Transcription factor 1
  Transcription factor 1 hepatic
  HNF1A_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/test

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated (ER1910-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Sample:
  HepG2 Cell (Human) Lysate at 30 ug
  Primary: Anti-HNF1 (Bs-1405R) at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 67 kD
  Observed band size: 67 kD

   

  

  Fig4: Blank control:A549.
  Primary Antibody (green line): Rabbit Anti-HNF1A antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 3μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！