Anti-HIPK1 antibody
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概述
- 产品描述May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.
- 产品名称Anti-HIPK1 antibody
- 分子量131 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,Macaque Monkey
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human HIPK1 1-100/1210
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus. Cytoplasm. Predominantly nuclear.
- 其它名称Hipk 1
Hipk1
HIPK1_HUMAN
Homeodomain interacting protein kinase 1
Homeodomain interacting protein kinase 1 like protein
Homeodomain-interacting protein kinase 1
Myak
Nbak 2
Nbak2
Nuclear body associated kinase 2
Nuclear body associated kinase 2b.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Mcf-7(Human) Cell Lysate at 40 ug
Primary: Anti-HIPK1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131kD
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