Anti-GPM6B antibody

Anti-GPM6B antibody

• 概述

• 产品描述May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.

• 产品名称Anti-GPM6B antibody

• 分子量29 KDa

• 种属反应性Human,Mouse,Rat,Pig,Horse

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GPM6B 1-100/265

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein (By similarity). Note=Colocalizes with SERT at the plasma membrane

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• ​其它名称M6b
  Neuronal membrane glycoprotein M6b
  rhombex 29
  GPM6B_HUMAN.

• 应用

  WB:1:500-2000
  FC:1μg/Test

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  Fig1: Sample:
  U251(Human) Cell Lysate at 30 ug
  Primary: Anti-GPM6B  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 29 kD
  Observed band size: 34 kD

   

  

  Fig2: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
  Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).

   

  

  Fig3: Sample: Brain (Mouse) Lysate at 40 ug
  Primary: Anti-GPM6B at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 29 kD
  Observed band size: 36 kD

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