Anti-GDF15 antibody

Anti-GDF15 antibody

• 概述

• 产品描述Growth/differentiation factor 15 (GDF15) was first identified as Macrophage inhibitory cytokine-1 or MIC-1. It is a protein belonging to the transforming growth factor beta superfamily. Under normal conditions, GDF-15 is expressed in low concentrations in most organs and upregulated because of injury of organs such as such as liver, kidney, heart and lung. The function of GDF-15 is not fully cleared but it seems to have a role in regulating inflammatory pathways and to be involved in regulating apoptosis, cell repair and cell growth, which are biological processes observed in cardiovascular and neoplastic disorders. GDF-15 has shown to be a strong prognostic protein in patients with different diseases such as heart diseases and cancer.

• 产品名称Anti-GDF15 antibody

• 分子量12.5/34 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GDF-15 221-308/308

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted.

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• ​其它名称GDF 15
  GDF-15 Growth differentiation factor 15
  Growth/differentiation factor 15
  Macrophage inhibitory cytokine 1
  MIC 1
  MIC-1
  MIC1
  NAG 1
  NAG1
  NRG 1
  NRG1
  NSAID (nonsteroidal anti inflammatory drug) activated protein 1
  NSAID
  NSAID regulated protein 1
  PDF
  PLAB
  Placental bone morphogenetic protein
  Placental bone morphogenic protein
  Placental TGF beta
  placental transformation growth factor beta
  Prostate differentiation factor
  PTGF beta
  PTGFB
  prostate derived factor.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

   

  

  Fig2: Sample:
  K562 Cell Lysate at 30 ug
  A431 Cell Lysate at 30 ug
  Primary: Anti-GDF15  at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size:34 kD
  Observed band size:30 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！