Anti-GALNT11 antibody

Anti-GALNT11 antibody

• 概述

• 产品描述Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward Muc1, Muc4.1, and EA2 than GALNT1. Does not appear to be involved in glycosylation of erythropoietin.

• 产品名称Anti-GALNT11 antibody

• 分子量69 KDa

• 种属反应性Human,Mouse,Rat,Dog,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GALNT11/GalNAc-T11 401-500/608

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Golgi apparatus membrane.
  

• 其它名称A430075I06Rik
  AI648252
  E430002F06Rik
  FLJ21634
  GalNAc-T11
  GALNT11
  GLT11_HUMAN
  MGC71630
  Polypeptide GalNAc transferase 11
  Polypeptide N-acetylgalactosaminyltransferase 11
  pp-GaNTase 11
  Protein-UDP acetylgalactosaminyltransferase 11
  tcag7.1057
  UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:Raji(human) Cell Lysate at 40 ug
  Primary: Anti-GALNT11at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 69kD
  Observed band size: 69kD

   

  

  Fig2: Sample:siha(human) Cell Lysate at 40 ug
  Primary: Anti-GALNT11at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 69kD
  Observed band size: 69kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GALNT11) Polyclonal Antibody, Unconjugated () at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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