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别名: | Collagen III | ||
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适用物种: | Human,Mouse,Rat,Cow | ||
验证应用: | WB,IHC-P,ICC/IF | ||
种属: | 兔多抗 | ||
储存条件: | -20℃ |
货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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熔点: | |
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密度: | |
储存条件: | -20℃ |
Anti-Collagen III antibody
产品描述Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (monomers), each called an alpha 1 chain of type III collagen. Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene. Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain. In addition to being an integral structural component of many organs, type III collagen is also an important regulator of the diameter of type I and II collagen fibrils. Type III collagen is also known to facilitate platelet aggregation through its binding to platelets and therefore, play an important role in blood clotting. Type III collagen is found as a major structural component in hollow organs such as large blood vessels, uterus and bowel. It is also found in many other tissues together with type I collagen.
产品名称Anti-Collagen III antibody
分子量117 KDa
种属反应性Human,Mouse,Rat,Cow
验证应用WB,IHC-P,ICC/IF
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain 801-900/1466
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Secreted, extracellular space, extracellular matrix.
其它名称
WB:1:500-2000
IHC-P:1:400-800
IF:1:100-500
Fig1: Sample:
Hela Cell (Human) Lysate at 40 ug
Primary: Anti- Collagen III at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 130 kD
Fig2: Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!