Anti-CHST7 antibody

Anti-CHST7 antibody

• 概述

• 产品描述CHST7 belongs to the sulfotransferase family. Sulfotransferases generate sulfated glycosaminoglycan (GAG) moities during chondroitin sulfate biosynthesis. They create considerable structural diversity among chondroitin sulfates by transferring sulfate with remarkable specificity for the underlying oligosaccharide substrate. This protein mainly transfers sulfate to N-acetylgalactosamine. The regulated expression of each member of the family may be an important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes.

• 产品名称Anti-CHST7 antibody

• 分子量54 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CHST7 101-200/486

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Golgi apparatus membrane; Single-pass type II membrane protein

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• ​其它名称6 O sulfotransferase 5
  C6ST 2
  C6ST2
  Carbohydrate sulfotransferase 7
  Chondroitin 6 sulfotransferase 2
  CHST 7
  CHST7_HUMAN
  CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7
  Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 5
  GlcNAc6ST 4
  Gn6st-4
  GST 5
  N acetylglucosamine 6 O sulfotransferase 1.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Ovary (Mouse) Lysate at 40 ug
  Primary: Anti- CHST7  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 54 kD
  Observed band size: 54 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！