Anti-CD33 antibody

Anti-CD33 antibody

• 概述

• 产品描述Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

• 产品名称Anti-CD33 antibody

• 分子量40/37 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse CD33 101-200/334

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Single-pass type I membrane protein.

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• ​其它名称CD33_HUMAN
  CD 33
  CD33 antigen
  CD33 molecule
  FLJ00391
  gp67
  Myeloid cell surface antigen CD33
  Myeloid cell surface antigen CD33 precursor
  p67
  Sialic acid binding Ig like lectin 3
  Sialic acid binding immunoglobulin like lectin 3
  SIGLEC3
  Siglec-3
  myeloid cell surface antigen CD33 isoform 1 precursor.

  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

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  Fig1: Blank control: mouse spleen cells (blue).
  Primary Antibody: Rabbit Anti- CD33 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  A549 Cell (Human) Lysate at 30 ug
  Primary: Anti-CD33  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 40/37 kD
  Observed band size: 40 kD

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