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| 别名: | Caspase-1 p20 | ||
|---|---|---|---|
| 适用物种: | Human, Mouse, Rat | ||
| 验证应用: | WB, FC | ||
| 种属: | 兔多抗 | ||
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ZY6905-47R-50 μl | 兔多抗 | 现货 | 1500 | ||||||||
| ZY6905-47R-100 μl | 兔多抗 | 现货 | 2500 | ||||||||
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Anti-Caspase-1 p20 antibody
产品描述Caspase-1/Interleukin-1 converting enzyme (ICE) is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, such as the precursors of the inflammatory cytokines interleukin 1β and interleukin 18 as well as the pyroptosis inducer Gasdermin D, into active mature peptides. It plays a central role in cell immunity as an inflammatory response initiator. Once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage and thus activation of the two inflammatory cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18) as well as pyroptosis, a programmed lytic cell death pathway, through cleavage of Gasdermin D. The two inflammatory cytokines activated by Caspase-1 are excreted from the cell to further induce the inflammatory response in neighboring cells. Activated Caspase 1 proteolytically cleaves pro IL-1β and pro IL-18 into their active forms, IL-1β and IL-18. The active cytokines lead to a downstream inflammatory response. It also cleaves Gasdermin D into its active form, which leads to pyroptosis. Once matured, the cytokines initiate downstream signaling events to induce a proinflammatory response as well as to activate the expression of antiviral genes. Following the inflammatory response, an activated Caspase-1 can induce pyroptosis, a lytic form of cell death, depending on the signal received as well as the specific inflammasome sensor domain protein that received it. Caspase-1 has also been shown to induce necrosis and may also function in various developmental stages.
产品名称Anti-Caspase-1 p20 antibody
分子量20/46 KDa
种属反应性Human, Mouse, Rat
验证应用WB, FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 181-280/402
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
亚型IgG
纯化方式Affinity purified by Protein A.
亚细胞定位Cytoplasm.
其它名称
WB:1:500-2000
FC:1μg/Test
Fig1: Sample:lung (rat) Lysate at 40 ug
Primary: Anti-Caspase-1 p20t 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/46kD
Observed band size: 46kD
Fig2: Sample:large intestine (rat) Lysate at 40 ug
Primary: Anti-Caspase-1 p20at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/46kD
Observed band size: 46kD
Fig3: Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody , Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC<,Dilution: 1μg /test.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
