Anti-Securin antibody
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概述
- 产品描述The product of the oncogene PTTG, pituitary tumor transforming gene, is a human homolog of the anaphase-inhibitor vertebrate protein, securin. PTTG contains a basic amino-terminal domain and an acidic carboxy-terminal domain, which acts as a transactivation domain when fused to a heterologous DNA binding domain. Human PTTG is overexpressed in Jurkat and is also detected in human thymus, testis and placenta. PTTG is mainly expressed in the cytoplasm and is also partially localized to the nucleus. Vertebrate PTTG regulates the separin Esp1, which promotes chromatid separation, to overcome the cohesive forces that hold sister chromatids together. This regulatory function of PTTG suggests that defective regulation of cohesion may contribute to cancer by promoting chromosome instability. Although vertebrate PTTG shares cell-cycle functions with its yeast securin counterparts Pds1p and Cut2, none share sequence homology.
- 产品名称Anti-Securin antibody
- 分子量22 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein with human Securin aa 1-200.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus. Cytoplasm.
- 其它名称AW555095 antibody
C87862 antibody
Cut2 antibody
EAP 1 antibody
EAP1 antibody
ESP1 associated protein 1 antibody
Esp1-associated protein antibody
hPTTG antibody
MGC126883 antibody
MGC138276 antibody
Pds1 antibody
Pituitary tumor transforming 1 antibody
Pituitary tumor transforming protein 1 antibody
Pituitary tumor-transforming 1, isoform CRA_a antibody
Pituitary tumor-transforming 1, isoform CRA_b antibody
Pituitary tumor-transforming gene 1 antibody
Pituitary tumor-transforming gene 1 protein antibody
PTTG 1 antibody
PTTG antibody
PTTG1 antibody
PTTG1 protein antibody
PTTG1_HUMAN antibody
Pttg3 antibody
Securin antibody
Tumor transforming 1 antibody
Tumor transforming protein 1 antibody
Tumor-transforming protein 1 antibody
TUTR 1 antibody
TUTR1 antibody
more
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应用
WB: 1:500
IHC-P: 1:200-1:500
ICC: 1:200-1:500
FC: 1:50-1:100
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Fig1: Western blot analysis of Securin on HUVEC cell lysate using anti-Securin antibody at 1/500 dilution.
Fig2: ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse tetis tissue using anti-Securin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Securin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of PC-3M cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
