Anti-AQP9 antibody

Anti-AQP9 antibody

• 概述

• 产品描述Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.
• 产品名称Anti-AQP9 antibody
• 分子量32 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human AQP9 201-295/295
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Multi-pass membrane protein.
• 其它名称aquaporin Protein-9
  Aquaporin 9
  AQP 9
  AQP9
  Aquaporin9
  HsT 17287
  HsT17287
  Small solute channel 1
  SSC 1
  SSC1
  Aquaporin-9
  AQP-9
  Aquaglyceroporin-9
  AQP9_HUMAN.
  more

• 应用

  WB:1:500-2000
  FC:1μg /test

•  

  

  Fig1: Sample:Intestinal(Mouse) lysate at 30 ug
  Primary: Anti-AQP9at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
  Predicted band size : 32 kD
  Observed band size : 32 kD

   

  

  Fig2: Sample:Liver(Mouse) lysate at 30 ug
  Primary: Anti-AQP9at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size : 32 kD
  Observed band size : 32 kD

   

  

  Fig3: Blank control: A549(blue).
  Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibodyDilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1903-85/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig4: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti-AQP9at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 32 kD
  Observed band size: 32 kD

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