Anti-alpha Adaptin antibody
-
概述
- 产品描述Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
- 产品名称Anti-alpha Adaptin antibody
- 分子量103 KDa
- 种属反应性Human,Mouse,Rat,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human alpha Adaptin 21-150/939
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
- 其它名称100 kDa coated vesicle protein A
100 kDa coated vesicle protein C
adaptin alpha A
Adaptin alpha B
Adaptor protein complex AP 2 alpha 1 subunit
Adaptor protein complex AP 2 alpha 2 subunit
Adaptor related protein complex 2 alpha 1 subunit
Adaptor related protein complex 2 alpha 2 subunit
ADTAA
ADTAB
Alpha adaptin A
Alpha adaptin C
AP 2 complex subunit alpha 1
AP 2 complex subunit alpha 2
AP2A2
CLAPA1
CLAPA2
Clathrin assembly protein complex 2 alpha A large chain
Clathrin assembly protein complex 2 alpha C large chain
Clathrin associated/assembly/adaptor protein large alpha 1
Clathrin associated/assembly/adaptor protein large alpha 2
HIP9
Huntingtin interacting protein HYPJ
Huntingtin interacting protein J
HYPJ
KIAA0899
Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
AP2A2_HUMAN
AP-2 complex subunit alpha-2
Adapter-related protein complex 2 alpha-2 subunit
Adaptor protein complex AP-2 subunit alpha-2
Alpha-adaptin C
Alpha2-adaptin
Clathrin assembly protein complex 2 alpha-C large chain
Huntingtin yeast partner J
Huntingtin-interacting protein 9
HIP-9
Huntingtin-interacting protein J
Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit.
more
-
应用
WB:1:500-2000
IHC-P:1:400-800
-
Fig1: Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-alpha Adaptin at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103 kD
Observed band size: 103 kD
Fig2: Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-alpha Adaptin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103 kD
Observed band size: 103 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (alpha Adaptin) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
