Anti-DOLPP1 antibody

Anti-DOLPP1 antibody

• 概述

• 产品描述DOLPP1 is a multi-pass membrane protein(By similarity). It belongs to the dolichyldiphosphatase family. It is required for efficient N-glycosylation and is necessary for maintaining optimal levels of dolichol-linked oligosaccharides. DOLPP1 hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. It does not act on phosphatidate
• 产品名称Anti-DOLPP1 antibody
• 分子量27 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human DOLPP1 151-238/238
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
• 其它名称0610011H20Rik
  AB030189
  DOPP1_HUMAN
  Dolichyldiphosphatase 1
  Linked to Surfeit genes in Fugu rubripes 2
  LSFR2
  OTTHUMP00000022326
  OTTHUMP00000196902.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-DOLPP1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 27 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DOLPP1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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