Anti-Cyclin B1 antibody

Anti-Cyclin B1antibody

• 概述

• 产品描述Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes.
• 产品名称Anti-Cyclin B1 antibody
• 分子量48 KDa
• 种属反应性Human,Mouse,Rat,Cow
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Cyclin B1 271-433/433
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
• 其它名称CCNB 1
  CCNB
  CCNB1
  CCNB1_HUMAN
  G2 mitotic specific cyclin B1
  G2/mitotic-specific cyclin-B1.

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

•  

  

  Fig1: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Cyclin B1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Cell: Hela
  Concentration:1:100
  Host/Isotype:Rabbit/IgG
  Flow cytometric analysis of primary antibody  on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

   

  

  Fig3: Sample:
  U251 Cell (Human) Lysate at 30 ug
  Primary: Anti- Cyclin B1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 50 kD

   

  

  Fig4: Sample:
  Hela Cell (Human) Lysate at 30 ug
  Primary: Anti- Cyclin B1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 50 kD

   

  

  Fig5: Sample:
  K562 Cell (Human) Lysate at 30 ug
  Primary: Anti- Cyclin B1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 50 kD

   

  

  Fig6: Sample:
  U937 Cell (Human) Lysate at 30 ug
  Primary: Anti- Cyclin B1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 50 kD

   

  

  Fig7: Paraformaldehyde-fixed, paraffin embedded (Rat esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 3

   

  

  Fig8: Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37

   

  

  Fig9: Blank control (blue line): A549 (blue).
  Primary Antibody (green line): Rabbit Anti-Cyclin B1 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): F(a

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