Anti-ERO1L antibody
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概述
- 产品描述Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
- 产品名称Anti-ERO1L antibody
- 分子量52 KDa
- 种属反应性Human,Mouse,Rat,Rabbit
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ERO1L 321-420/468
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
- 其它名称Endoplasmic oxidoreductin 1 like protein
Endoplasmic oxidoreductin-1-like protein
ERO1 alpha
ERO1 L
ERO1 Lalpha
ERO1 like protein alpha
ERO1-L
ERO1-L-alpha
ERO1-like (S. cerevisiae)
ERO1-like protein alpha
ERO1A
ERO1A_HUMAN
ERO1L
Oxidoreductin 1 Lalpha
Oxidoreductin-1-L-alpha.
more
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Fig1: Sample:
SW480(Human) Cell Lysate at 40 ug
Primary: Anti-ERO1Lat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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