Anti-EphA2 antibody
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概述
- 产品描述Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
- 产品名称Anti-EphA2 antibody
- 分子量105 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Monkey
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human EphA2 901-976/976
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Note=Present at regions
- 其它名称ARCC2
Eck
Eph receptor A2 antibody EphA2
Ephrin receptor
Ephrin receptor EphA2
Ephrin type A receptor 2
Epithelial cell kinase
Epithelial cell receptor protein tyrosine kinase antibody Myk 2
Myk2
Sek 2
Sek2
Tyrosine protein kinase receptor ECK
Tyrosine-protein kinase receptor MPK-5
Tyrosine-protein kinase receptor SEK-2
EPHA2_HUMAN.
more
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Fig1: Sample:
Human colon carcinoma lysates Lysate at 30 ug
Intestine (Mouse) Lysate at 30 ug
Primary: Anti- EphA2/Eph receptor A2 at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size : 105kD
Observed band size : 105kD, 52/55kD
Fig2: Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-EphA2 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kD
Observed band size: 115 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
