Anti-Apg7 antibody
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概述
- 产品描述In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.
- 产品名称Anti-Apg7 antibody
- 分子量78 kDa
- 种属反应性Human,Mouse
- 验证应用ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm.
- 其它名称1810013K23Rik antibody
Apg 7 antibody
APG7 autophagy 7 like antibody
APG7 autophagy 7-like (S. cerevisiae) antibody
APG7 like antibody
APG7, S. cerevisiae, homolog of antibody
APG7-like antibody
APG7L antibody
ATG 7 antibody
ATG12-activating enzyme E1 ATG7 antibody
ATG7 antibody
ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
ATG7 autophagy related 7 homolog antibody
ATG7_HUMAN antibody
Atg7l antibody
Autophagy 7, S. cerevisiae, homolog of antibody
Autophagy related protein 7 antibody
Autophagy-related 7 (yeast) antibody
Autophagy-related protein 7 antibody
DKFZp434N0735 antibody
GSA 7 antibody
GSA7 antibody
hAGP7 antibody
Ubiquitin activating enzyme E1 like protein antibody
Ubiquitin-activating enzyme E1-like protein antibody
Ubiquitin-like modifier-activating enzyme ATG7 antibody
more
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应用
ICC: 1:500-1:2,000
IHC-P: 1:200-1:1,000
FC: 1:50-1:100
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Fig1: ICC staining Apg7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining Apg7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Apg7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Jurkat cells with Apg7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!