Anti-FOXO3A antibody
-
概述
- 产品描述Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
- 产品名称Anti-FOXO3A antibody
- 分子量71 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称AF6q21 antibody
AF6q21 protein antibody
DKFZp781A0677 antibody
FKHR2 antibody
FKHRL 1 antibody
FKHRL1 antibody
FKHRL1P2 antibody
Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
Forkhead box O3 antibody
Forkhead box O3A antibody
Forkhead box protein O3 antibody
Forkhead box protein O3A antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
Forkhead homolog (rhabdomyosarcoma) like 1 antibody
Forkhead in rhabdomyosarcoma like 1 antibody
Forkhead in rhabdomyosarcoma-like 1 antibody
FOX O3A antibody
FOXO2 antibody
foxo3 antibody
FOXO3_HUMAN antibody
FOXO3A antibody
MGC12739 antibody
MGC31925 antibody
more
-
应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of FOXO3A on different cell lysate using anti-FOXO3A antibody at 1/500 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: K562
Fig2: ICC staining FOXO3A in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining FOXO3A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining FOXO3A in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SHSY5Y cells with FOXO3A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the seconda
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
