Anti-ULK1 antibody
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概述
- 产品描述ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 maps to human chromosome 12q24.3 and is ubiquitously expressed. ULK2, also widely expressed, maps to mouse chromosome 11B1.3 and is expected to have a similar molecular weight as ULK1 in human. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes (1,2,6).
- 产品名称Anti-ULK1 antibody
- 分子量113 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用ICC,IHC-P,FC,WB
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytosol.
- 其它名称ATG 1 antibody
ATG1 antibody
ATG1 autophagy related 1 homolog antibody
ATG1A antibody
Autophagy related protein 1 homolog antibody
Autophagy-related protein 1 homolog antibody
FLJ38455 antibody
FLJ46475 antibody
hATG1 antibody
KIAA0722 antibody
Serine/threonine protein kinase ULK1 antibody
Serine/threonine protein kinase Unc51.1 antibody
Serine/threonine-protein kinase ULK1 antibody
ULK 1 antibody
ULK1 antibody
ULK1_HUMAN antibody
Unc 51 (C. elegans) like kinase 1 antibody
UNC 51 antibody
Unc 51 like kinase 1 antibody
Unc-51 like kinase 1 (C. elegans) antibody
Unc-51-like kinase 1 antibody
UNC51 antibody
UNC51, C. elegans, homolog of antibody
Unc51.1 antibody
more
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应用
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
WB: 1:500
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Fig1: ICC staining ULK1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining ULK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ULK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SH-SY5Y cells with ULK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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