Anti-DDIT4 antibody
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概述
- 产品描述REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.
- 产品名称Anti-DDIT4 antibody
- 分子量25 kDa (Predicted band size）
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Mitochondrion. Cytosol.
- 其它名称DDIT4 antibody
DDIT4_HUMAN antibody
Dig2 antibody
DNA damage inducible transcript 4 antibody
DNA damage inducible transcript 4 protein antibody
DNA damage-inducible transcript 4 protein antibody
FLJ20500 antibody
HIF 1 responsive protein RTP801 antibody
HIF 1 responsive RTP801 antibody
HIF-1 responsive protein RTP801 antibody
Protein regulated in development and DNA damage response 1 antibody
REDD-1 antibody
REDD1 antibody
RTP801 antibody
more
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应用
WB: 1:500
ICC: 1:50-1:100
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.
Fig2: ICC staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of A549 cells with DDIT4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
