Anti-FOXM1 antibody
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概述
- 产品描述The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13. The transcription element that restricts FOXM1 expression to proliferating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B.
- 产品名称Anti-FOXM1 antibody
- 分子量84 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus.
- 其它名称FKHL16 antibody
Forkhead box M1 antibody
Forkhead box protein M1 antibody
forkhead like 16 antibody
Forkhead-related protein FKHL16 antibody
FOX M1 antibody
Foxm1 antibody
FOXM1_HUMAN antibody
FOXM1B antibody
Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
HFH-11 antibody
HFH11 antibody
HNF-3/fork-head homolog 11 antibody
HNF3 antibody
INS1 antibody
M phase phosphoprotein 2 antibody
M-phase phosphoprotein 2 antibody
MPHOSPH2 antibody
MPM-2 reactive phosphoprotein 2 antibody
MPP2 antibody
PIG29 antibody
TGT3 antibody
Transcription factor Trident antibody
Trident antibody
WIN antibody
Winged-helix factor from INS-1 cells antibody
Winged-helix factor from INS1 cells antibody
more
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应用
ICC: 1:500-1:2,000
FC: 1:50-1:100
WB: 1:500
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Fig1: ICC staining FOXM1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining FOXM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining FOXM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Flow cytometric analysis of LOVO cells with FOXM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
