Anti-STEP antibody

Anti-STEP antibody

• 概述

• 产品描述May regulates the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
• 产品名称Anti-STEP antibody
• 分子量63 KDa
• 种属反应性Human,Mouse,Rat,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human PTPN5 201-300/565
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cellular localizationEndoplasmic reticulum membrane.
• 其它名称PTPN5
  Neural specific protein tyrosine phosphatase
  Neural-specific protein-tyrosine phosphatase
  Protein tyrosine phosphatase non receptor type 5 (striatum enriched)
  Protein tyrosine phosphatase non receptor type 5
  Protein tyrosine phosphatase striatum enriched
  PTN5
  PTN5_HUMAN
  PTP STEP
  PTPN 5
  Ptpn5
  PTPSTEP
  Striatum-enriched protein-tyrosine phosphatase
  Tyrosine protein phosphatase non receptor type 5
  Tyrosine-protein phosphatase non-receptor type 5
  FLJ14427.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  HepG2(Human) Cell Lysate at 30 ug
  U2OS(Human) Cell Lysate at 30 ug
  Primary: Anti- STEP at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 63 kD
  Observed band size: 63 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (STEP) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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