Anti-C3a Receptor antibody

Anti-C3a Receptor antibody

• 概述

• 产品描述Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
• 产品名称Anti-C3a Receptor antibody
• 分子量54 KDa
• 种属反应性Human,Mouse,Rat,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR 401-482/482
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Multi-pass membrane protein.
• 其它名称AZ 3B
  AZ3B
  C3a anaphylatoxin chemotactic receptor
  C3A R
  C3A R1
  C3a-R
  C3AR
  C3AR_HUMAN
  C3AR1
  C3R1
  Complement component 3 receptor 1
  Complement component 3a receptor 1
  HNFAG09.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (ER1904-90) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  K562(Human) Cell Lysate at 30 ug
  293T(Human) Cell Lysate at 30 ug
  A549(Human) Cell Lysate at 30 ug
  Lung (Mouse) Lysate at 40 ug
  Primary: Anti-C3a Receptorat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 54 kD
  Observed band size: 64 kD

   

  

  Fig3: Sample: A549 Cell (Human) Lysate at 30 ug
  Primary: Anti-C3a Receptoat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 54kD
  Observed band size: 60kD

   

  

  Fig4: Sample: 293T Cell (Human) Lysate at 30 ug
  Primary: Anti-C3a Recepto 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 54kD
  Observed band size: 60kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！