Anti-FNDC3A antibody

Anti-FNDC3A antibody

• 概述

• 产品描述Fibronectin type III domain containing 7, or, FNDC7, is a human gene. Other names include: Fibronectin type III domain-containing protein 7. The encoded protein has an amino acid length of 733 and a mass of 78.2 kDa. Other isoforms may exist. This gene shares homology with other species, including: Mouse, Rat, Zebrafish, Frog, Chicken.
• 产品名称Anti-FNDC3A antibody
• 分子量67 kDa
• 种属反应性Zebrafish
• 验证应用IF,IHC-P
• 抗体类型兔多抗
• 免疫原Recombinant protein within Zebrafish FNDC3A aa 100-310.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein affinity purified.
• 亚细胞定位Golgi apparatus, Membrane.
• 其它名称Fibronectin type III domain containing protein 3a antibody
  FNDC3 antibody
  HUGO antibody
  KIAA0970 antibody

• 应用

  IF:1:50-1:200
  IHC-P:1:50-1:200

•  

  

  Fig1: Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-66) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-66) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

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