Anti-HEC1 antibody
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概述
- 产品描述Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
- 产品名称Anti-HEC1 antibody
- 分子量74 kDa
- 种属反应性Human
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human HEC1 300-500 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus.
- 其它名称from Entrez Gene ; antibody
HEC antibody
Highly expressed in cancer antibody
Highly expressed in cancer protein antibody
Highly expressed in cancer rich in leucine heptad repeats antibody
HsHec1 antibody
hsNDC80 antibody
Kinetochore associated 2 antibody
Kinetochore associated protein 2 antibody
Kinetochore protein Hec1 antibody
Kinetochore protein NDC80 homolog antibody
Kinetochore-associated protein 2 antibody
KNTC2 antibody
ndc80 antibody
NDC80 homolog kinetochore complex component antibody
NDC80 kinetochore complex component homolog antibody
NDC80, S. cerevisiae, homolog of antibody
NDC80_HUMAN antibody
Retinoblastoma associated protein HEC antibody
Retinoblastoma-associated protein HEC antibody
TID3 antibody
more
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应用
WB: 1:500
FC: 1:50-1:100
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Fig1: Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution.
Positive control:
Lane 1, 3: Anti-HEC1 antibody
Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
Fig2: Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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