Anti-IL-5 antibody

Anti-IL-5 antibody

• 概述

• 产品描述Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion - primarily IgA. It is also a key mediator in eosinophil activation. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease.
• 产品名称Anti-IL-5 antibody
• 分子量13 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC/IF
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from mouse IL-5 56-132/132
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称B Cell Differentiation Factor I
  IL5
  IL 5
  Colony stimulating factor
  Colony Stimulating Factor Eosinophil
  EDF
  Eosinophil
  Eosinophil Differentiation Factor
  IL 5
  Interleukin 5
  B-cell differentiation factor I
  IL-5
  IL5_RAT
  Interleukin-5
  T Cell Replacing Factor
  T-cell replacing factor
  Interleukin5
  TRF.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Sample:
  Lovo Cell (Human) Lysate at 30 ug
  Primary: Anti- IL-5at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 13kD
  Observed band size: 10kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！