Anti-Alpha smooth muscle Actin antibody
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概述
- 产品描述Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
- 产品名称Anti-Alpha smooth muscle Actin antibody
- 分子量42 kDa
- 种属反应性Human,Mouse,Rat, Xenopus laevis, Chicken, Cow, Horse, Monkey
- 验证应用WB, ICC, IHC-P
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoskeleton
- 其它名称a-SMA antibody
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ACTA_HUMAN antibody
ACTA2 antibody
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Actin aortic smooth muscle antibody
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ACTSA antibody
ACTVS antibody
Alpha 2 actin antibody
Alpha actin 2 antibody
Alpha cardiac actin antibody
Alpha-actin-2 antibody
Cell growth inhibiting gene 46 protein antibody
Cell growth-inhibiting gene 46 protein antibody
GIG46 antibody
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应用
WB: 1:2,000
ICC: 1:200
IHC-P: 1:200
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Fig1: Western blot analysis of alpha smooth muscle actin on different cell lysates using anti-Alpha smooth muscle actin antibody at 1/2000 dilution.
Positive control:
Lane 1: Jurkat
Lane2 : NIH/3T3
Lane 3: Mouse embryonic stem cell
Lane 4: Mouse colon
Fig2: ICC staining of Alpha smooth muscle actin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining of Alpha smooth muscle actin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Alpha smooth muscle actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Alpha smooth muscle actin antibody. Counter stained with hematoxylin.
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