- Product Name
- Human CH25H (Cholesterol 25-hydroxylase) ELISA KIT
- Catalogue No.
- ZY14188EH
- Alias
- Cholesterol 25-hydroxylase| H25oh| CH25H ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- CH25H ELISA KIT allows for the in vitro quantitative determination of CEMIP concentrations in plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.156-10ng/ml
- Sensitivity
- < 0.094ng/ml
- Species
- Human
- Standard Curve
- Storage
- 4 ℃ for 6 months
- Recovery
-
Matrices listed below were spiked with certain level of CEMIP and the recovery rates were calculated by comparing the measured value to the expected amount of CEMIP in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 91-100 97 EDTA plasma(n=5) 89-95 92 heparin plasma(n=5) 86-105 97 - Linearity
-
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEMIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 87-105% 85-100% 89-105% 95-104% EDTA plasma(n=5) 86-99% 86-101% 84-94% 85-96% heparin plasma(n=5) 80-95% 82-97% 81-96% 82-94% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only
-
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