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异柠檬酸脱氢酶1(IDH1)重组蛋白(Mouse,小鼠)

Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
别名: PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
储存条件: 在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。 
异柠檬酸脱氢酶1(IDH1)重组蛋白(Mouse,小鼠)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY66073MuP-10μg 10μg 现货 1056.00 1056
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熔点:
密度:
储存条件: 在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

异柠檬酸脱氢酶1(IDH1)重组蛋白

规格及价格:

规格 价格
10µg 1056
50µg 2640
200µg 5280
1mg                  15840
[ PROPERTIES ]
Host: E.coli
Residues: Ile99~Thr325 ,with two N-terminal Tags, His-tag and GST-tag.
Subcellular Location: Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 57.6kDa
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-IR NILGGTVFRE AIICKNIPRLVTGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPKD GTQKVTYMVH DFEEGGGVAMGMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFEAQKICYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGMMTSVLICPDGKTVEAEAAHG TVTRHYRMYQ KGQET
[ REFERENCES ]
1. Nekrutenko A., et al. (1998) Mol. Biol. Evol. 15:1674-1684.
2. Carninci P., et al. (2005) Science 309:1559-1563.
3. Park J., et al. (2013) Mol. Cell 50:919-930.
4. Rardin M.J., et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606.
[ IDENTIFICATION ]
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